Study of hepatocyte plasma membrane mechanical properties using optical trapping

16th Russian Youth Conference on Physics and Astronomy

物理学;天文学

Vedyaykin, A.D.^1 ; Morozova, N.E.^1 ; Pobegalov, G.E.^1 ; Arseniev, A.N.^1 ; Khodorkoskii, M.A.^1 ; Sabantsev, A.V.^1

St. Petersburg State Polytechnical University, Institute of Nanobiotechnologies, St. Petersburg, Russia^1

关键词: Direct measurement;    Hepatocellular carcinoma;    Human hepatocytes;    Mechanical parameters;    Membrane mechanical properties;    Membrane tethers;    Membrane viscosity;    Parameters characterizing;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/572/1/012016/pdf
DOI  :  10.1088/1742-6596/572/1/012016

学科分类：天文学(综合)

来源: IOP

【 摘 要 】

In this paper we describe the use of membrane tether formation technique which is widely used to study mechanical properties of plasma membranes. This method was successfully used for the direct measurement of parameters characterizing membranes mechanical properties (static tether tension force and effective membrane viscosity) of human hepatocytes (HepG2 hepatocellular carcinoma line). These results allow using this method in future for diagnostics of the cell membrane, evaluating the influence on the mechanical parameters of various factors, including toxins and drugs.