Preparation and properties of magnetic-fluorescent endoglin aptamer nanoprobe

2018 International Conference on Advanced Materials, Intelligent Manufacturing and Automation

材料科学;机械制造;运输工程

Zhou, Zhide^1 ; Wang, Zhihong^1 ; Zhao, Le^1 ; Li, Guiyin^1

School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi

541004, China^1

关键词: Average particle size;    Early diagnosis;    Endoglin;    Fourier transform infrared;    Hepatocellular carcinoma;    Magnetic functions;    Molecular Probes;    Photoluminescence spectrum;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/382/2/022035/pdf
DOI  :  10.1088/1757-899X/382/2/022035

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

In this study, a kind of magnetic-fluorescent endoglin aptamer nanoprobe, Cy5.5-End-Fe3O4/KCTS, was synthesized with magnetic functions of Fe3O4/KCTS nanoparticles and the fluorescence ability of Cy5.5 through SMCC cross-linking with endoglin aptamer labeled with Cy5.5. The microstructure, fluorescence and magnetic properties of the samples were investigated and discussed by Fourier transform-infrared (FT-IR) spectra, X-ray diffraction (XRD), transmission electron microscopy (TEM), photoluminescence spectra, dynamic light scattering (DLS) and Zeta potential. The magnetic-fluorescent aptamer nanoprobe has an average particle size of about 240 nm, Zeta potential of -8.9 mV, excellent fluorescence and magnetic properties. This study provides a new MRI/fluorescent bimodal molecular probe for early diagnosis of hepatocellular carcinoma.