Interleukin-6-174 promoter polymorphism and susceptibility to hepatitis B virus infection in Javanese individuals

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Prasetyo, A.A.^1,2 ; Marwoto, M.^1 ; Monica, W.^1

A-IGIC (A-Infection Genomic Immunology and Cancer), Research Group, Sumber, Banjarsari, Surakarta

57138, Indonesia^1

Department of Microbiology, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^2

关键词: Healthy individuals;    Hepatitis B virus;    Hepatitis B virus infection;    Hepatocellular carcinoma;    Humoral immune response;    Peripheral blood;    Polymerase chain reaction methods;    Specific primers;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012114/pdf
DOI  :  10.1088/1757-899X/434/1/012114

来源: IOP

【 摘 要 】

Hepatitis B virus infection is a significant risk factor for cirrhosis and hepatocellular carcinoma. Interleukin-6, a cytokine with broad pleiotropic actions, plays important role in cellular and humoral immune responses to viral infection. The -174 G/C polymorphisms of interleukin-6 is a frequent polymorphism and may determine the outcome of viral infection. To find out the genetic association between the -174 G/C polymorphism and susceptibility to hepatitis B virus infection in Javanese individuals, blood samples from Javanese hepatitis B virus-infected individuals and from Javanese healthy individuals confirmed by serological and molecular assays were evaluated. Genomic DNA was extracted from peripheral blood and the sequence specific primer-polymerase chain reaction method was applied for genotyping. The frequencies of genotypes C/C, C/G and G/G in hepatitis B virus-infected samples were 5.3%, 31.6%, 63.1% and in controls were 20%, 38%, and 42%, respectively. The frequencies of G allele in hepatitis B virus-infected samples was higher than that of healthy samples (OR 2.4, 95%CI: 1.289-4.459, p = 0.0057). These findings suggest that the G allele was associated with susceptibility to hepatitis B virus infection in Javanese individuals.