Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos

STAR Protocols

Rena Hatakeyama¹  Kei Miyamoto²  Christopher A. Penfold³  Junko Tomikawa⁴ 

[1] Centre for Trophoblast Research, University of Cambridge, Downing Site, CB2 3EG Cambridge, UK;Wellcome Trust – Medical Research Council Stem Cell Institute, University of Cambridge, Jeffrey Cheah Biomedical Centre, Puddicombe Way, CB2 0AW Cambridge, UK;Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Site, CB2 3EG Cambridge, UK;Laboratory of Molecular Developmental Biology, Faculty of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan;

关键词: Bioinformatics;    Cell Biology;    Cell culture;    Developmental biology;    Molecular Biology;    Gene Expression;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Reprogramming of somatic nuclei toward the embryonic state has been studied using nuclear transfer (NT) to an oocyte at the metaphase II (MII) stage. In this NT, a somatic nucleus transplanted into an MII oocyte of the same species undergoes DNA replication and cell division before activating embryonic genes. Here, we describe a direct NT protocol using 4-cell stage mouse embryos that enables reprogramming of intra- and cross-species nuclei to express embryonic genes without requiring DNA replication and cell division.For complete details on the use and execution of this protocol, please refer to Tomikawa et al. (2021).

【 授权许可】

Unknown