Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction

STAR Protocols

Yoshiki Iemura¹  Yasuhiro Takashima²  Shingo Io³ 

[1] Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan;Department of Obstetrics and Gynecology, Kosaka Women’s Hospital, Osaka 577-0807, Japan;Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan;

关键词: Cell Biology;    Cell culture;    Cell Differentiation;    Developmental biology;    Flow Cytometry/Mass Cytometry;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Human trophoblasts arise from the morula as trophectoderm, which differentiates into cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast after implantation. Here, we present a robust step-by-step protocol to induce trophectoderm (TE) from naive human pluripotent stem cells (PSCs) corresponding to pre-implantation epiblast. Our culture system (TE induction and ACE condition) mimics the entire trophoblast development including the molecular events.For complete details on the use and execution of this protocol, please refer to Io et al. (2021).

【 授权许可】

Unknown