Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells

STAR Protocols

卷:2

Eddie Spencer Qian¹  Julie Bejoy²  Lauren Elizabeth Woodard² 

[1] Corresponding author;

[2] Department of Medicine, Division of Nephrology and Hypertension, Vanderbilt University Medical Center, Nashville, TN 37232, USA;

关键词: Cell culture;    Developmental biology;    Stem Cells;    Cell Differentiation;    Tissue Engineering;    Biotechnology and bioengineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Several kidney diseases including congenital nephrotic syndrome, Alport syndrome, and diabetic nephropathy are linked to podocyte dysfunction. Human podocytopathies may be modeled in either primary or immortalized podocyte cell lines. Human induced pluripotent stem cell (hiPSC)-derived podocytes are a source of human podocytes, but the existing protocols have variable efficiency and expensive media components. We developed an accelerated, feeder-free protocol for deriving functional, mature podocytes from hiPSCs in only 12 days, saving time and money compared with other approaches.

【 授权许可】

Unknown