Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells

STAR Protocols

Juan Carlos Izpisua Belmonte¹  Ronghui Li² 

[1] Corresponding author;Salk Institute for Biological Studies, La Jolla, CA 92037, USA;

关键词: Cell Biology;    Cell culture;    Developmental biology;    Stem Cells;    Cell Differentiation;    Organoids;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis.For complete details on the use and execution of this protocol, please refer to Li et al. (2019).

【 授权许可】

Unknown