Analyzing mouse neural stem cell and progenitor cell proliferation using EdU incorporation and multicolor flow cytometry

STAR Protocols

Ekta Kumari¹  Krista D. Buono²  Fernando Janczur Velloso²  Steven W. Levison²  Michelle J. Frondelli³ 

[1] Corresponding author;Department of Pharmacology, Physiology and Neurosciences, Rutgers-NJMS, Newark, NJ 07103, USA;ICON Laboratory Services, Farmingdale, NY 11735, USA;

关键词: Cell Biology;    Cell isolation;    Flow Cytometry/Mass Cytometry;    Developmental biology;    Neuroscience;    Stem Cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell surface antigens to distinguish between 8 phenotypically distinct mouse neural progenitors and stem cells. It has been optimized for wild-type neonatal mice but can be used on mice of any postnatal age.For complete details on the use and execution of this profile, please refer to Kumari et al. (2020).

【 授权许可】

Unknown