Isolation of genetically manipulated neural progenitors and immature neurons from embryonic mouse neocortex by FACS

STAR Protocols

Yukiko Gotoh¹  Yusuke Kishi² 

[1] Corresponding author;Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;

关键词: Cell Biology;    Cell isolation;    Flow Cytometry/Mass Cytometry;    Developmental biology;    Molecular Biology;    Gene Expression;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The embryonic mammalian neocortex includes neural progenitors and neurons at various stages of differentiation. The regulatory mechanisms underlying multiple aspects of neocortical development—including cell division, neuronal fate commitment, neuronal migration, and neuronal differentiation—have been explored using in utero electroporation and virus infection. Here, we describe a protocol for investigation of the effects of genetic manipulation on neural development through direct isolation of neural progenitors and neurons from the mouse embryonic neocortex by fluorescence-activated cell sorting.For complete details on the use and execution of this protocol, please refer to Tsuboi et al. (2018) and Sakai et al. (2019).

【 授权许可】

Unknown