Generation of precision-cut slice cultures of human placenta

STAR Protocols

卷:3

Blandina I. Oliveira Esteves¹  Marco P. Alves²  Melanie Brügger²  Beatrice Zumkehr³  Amal Fahmi⁴  David Baud⁴ 

[1] Corresponding author;

[2] Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland;

[3] Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland;

[4] Institute of Virology and Immunology, Bern, Switzerland;

关键词: Cell Biology;    Cell culture;    Developmental biology;    Health Sciences;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease.For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021).

【 授权许可】

Unknown