Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells

Stem Cell Reports

Richard Shek-Kwan Chang¹  Graham Ka-Hon Shea²  Qiang Ao³  Sa Cai⁴  Daisy Kwok-Yan Shum⁴  Kin-Wai Tam⁴  Ying-Shing Chan⁴  Yat-Ping Tsui⁴ 

[1] Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR;Department of Orthopaedics & Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR;Department of Tissue Engineering, China Medical University, Shenyang, PR China;School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR;

关键词: bone marrow stromal cell;    Schwann cell;    differentiation;    fate commitment;    myelination;   

DOI  :  10.1016/j.stemcr.2017.08.004

来源: DOAJ

【 摘 要 】

Our ultimate goal of in vitro derivation of Schwann cells (SCs) from adult bone marrow stromal cells (BMSCs) is such that they may be used autologously to assist post-traumatic nerve regeneration. Existing protocols for derivation of SC-like cells from BMSCs fall short in the stability of the acquired phenotype and the functional capacity to myelinate axons. Our experiments indicated that neuro-ectodermal progenitor cells among the human hBMSCs could be selectively expanded and then induced to differentiate into SC-like cells. Co-culture of the SC-like cells with embryonic dorsal root ganglion neurons facilitated contact-mediated signaling that accomplished the switch to fate-committed SCs. Microarray analysis and in vitro myelination provided evidence that the human BMSC-derived SCs were functionally mature. This was reinforced by repair and myelination phenotypes observable in vivo with the derived SCs seeded into a nerve guide as an implant across a critical gap in a rat model of sciatic nerve injury.

【 授权许可】

Unknown