Cytotoxicity and genotoxicity of GO-Fe3O4 hybrid in cultured mammalian cells

Polish Journal of Chemical Technology

Jedrzejczak-Silicka Magdalena¹ 

[1] West Pomeranian University of Technology, Szczecin, Laboratory of Cytogenetics, DoktoraJudyma 6, 71-466, Szczecin, Poland;

关键词: go-fe3o4 hybrid;    relative viability;    dna fragmentation;    micronucleus assay;    genotoxicity;   

DOI  :  10.1515/pjct-2017-0004

来源: DOAJ

【 摘 要 】

The study was aimed at investigating the effect of the Fe3O4 hybrid deposited on graphene oxide (GO-Fe3O4) on the relative viability and DNA integrity. The properties of the GO-Fe3O4 hybrid were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) and thermal gravimetric method (TGA), while the efficiency of graphene oxide covalent functionalization with iron oxide nanospheres was determined by Fourier transform infrared spectroscopy (FT-IR). L929 and MCF-7 cell lines were selected to analyze the biocompatibility of GO-Fe3O4 nanoparticles. The hybrid was tested using WST-1 and LDH leakage assays. DNA integrity was analyzed by agarose gel electrophoresis and micronucleus assay was performed to examine chromosomal damage in the exposed cell lines. The tested GO-Fe3O4 hybrid did not significantly reduce cell metabolism of L929 cells. GO-Fe3O4 hybrid particles only slightly affected the integrity of cell membranes. DNA integrity and micronucleus assays did not indicate genotoxicity of the hybrid.

【 授权许可】

Unknown