Correlation between Different Patterns of Hypo-Osmotic Swelling and Sperm Functional Tests

International Journal of Fertility and Sterility

Mohammad Hossein Nasr Esfahani¹  Farzaneh Bassiri¹  Marziyeh Tavalaee¹ 

[1] Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute forBiotechnology, ACECR, Isfahan, Iran;

关键词: icsi;    host;    dna fragmentation;    protamine;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background:Sperm membrane integrity is not only important as a barrier between intra-and extra-cellular spaces, but also it can be considered as a sign of DNA integrity. Hypoosmotic swelling test reflects membrane integrity and has been used to evaluate spermquality. Intracytoplasmic sperm injection (ICSI) in adjunct with hypo-osmotic swellingtest (HOST) has been used for treatment of males with asthenozoospermia. Therefore,this study aims to evaluate correlation of different pattern of HOST with sperm parameters, protamine deficiency and apoptosis.Materials and Methods:In this case-control study, sixteen semen samples were randomly collected from infertile normozospermic men. Semen samples were divided intotwo portions as follows: one portion was assessed for sperm parameters according toWorld Health Organization (WHO)-2010, while the other portion, after applying HOSTprocedure, was used for assessment of sperm morphology, protamine deficiency and lateor early apoptosis. Statistical analysis was carried out using the Statistical Package forthe Social Studies (SPSS 11.5).Results:Our results showed that, the lowest odds ratio (OR) of abnormal sperm headmorphology and abnormal acrosome was in d-sperm as compared to a-pattern or nonviable spermatozoa (p=0.00, p=0.01). In addition, a significant correlation was observedbetween d-sperm with sperm concentration and percentage of DNA damage (p=0.03and p=0.04, respectively). A significant correlation was observed between percentageof sperm motility and DNA fragmentation (r=-0.56; p=0.01). Furthermore, significantcorrelations were observed between percentages of early apoptotic sperm with protaminedeficiency and sperm concentration (p=0.009 and p=0.01, respectively).Conclusion:Significant correlations exist between d-pattern and sperm DNA integrity.Semen samples with low sperm concentration have low percentage of d-sperm which aremature and intact sperms.

【 授权许可】

Unknown