Increased Sperm DNA Damage in Experimental Rat Varicocele Model and The Beneficial Effect of Varicocelectomy

International Journal of Fertility and Sterility

Muhammet Ihsan Karaman¹  Metin İshak Öztürk¹  Muzaffer Oğuz Keleş¹  Orhan Koca¹  Seda Yılmaz² 

[1] Department of Urology, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey;Gebze Institute of High Technology Molecular Biology and Genetics, Kocaeli, Turkey;

关键词: varicocele;    sperm;    dna fragmentation;    sperm chromatin dispersion test;   

DOI  :  

来源: DOAJ

【 摘 要 】

BackgroundVaricocele, the abnormal dilatation of the veins in the pampiniform plexus is commonly seen in infertile patients. In this study, we aim to examine sperm DNAdamage after the creation of experimental varicocele in rats and to observe the changeof this damage after a varicocelectomy.Materials and MethodsIn this experimental study, a total of 30 adult male Wistar albinorats were divided into three groups. The 10 rats in group 1 underwent a sham operation, anexperimental varicocele was created in both the10 rats in group 2 and the 10 rats in group 3 (atotal of 20 rats). While the rats of group 2 were sacrificed after four weeks, the rats in group 3underwent a varicocelectomy after four weeks and were sacrificed four weeks after the varicocelectomy to observe its effects. Sperm DNA fragmentation was assessed with a Halomax®kit. The DNA Fragmentation Index (DFI) was calculated and the groups were compared according to their DFI. Statistical analysis was performed using the Mann-Whitney U test.ResultsMedian sperm DFI was 17.6 (range: 7.6) in the right testicle and 18.3 (range: 6.8)in the left testicle in the control group; 30.7 (range: 8.8) in the right testicle and 31.8 (range:9.6) in the left testicle in the varicocele group; 27.1 (range: 8.1) in the right testicle and 28.6(range: 8.9) in the left testicle in the varicocelectomy group. DNA damage in both right andleft testicles was statistically significant between the three groups (p < 0.05).ConclusionThe results of this study show that varicocele leads to increased spermDNA damage and this damage is decreased by varicocelectomy.

【 授权许可】

Unknown