Can Altered Expression of HSPA2 in Varicocele Patients Lead to Abnormal Spermatogenesis?

International Journal of Fertility and Sterility

Farzad Rajaei¹  Farzaneh Zamansoltani¹  Shahnaz Razavi²  Nazem Ghasemi³  Kamran Ghaedi⁴  Somayeh Tanhaei⁵  Mohammad Hossein Nasr Esfahani⁶  Mohammad Reza Deemeh⁶  Marziyeh Tavalaee⁶  Zahra Mirhosseini⁶  Homayoun Abbasi⁷ 

[1] Department of Anatomy, Ghazvin Medical University, Ghazvin, Iran;Department of Anatomy, Isfahan Medical University, Isfahan, Ira;Department of Anatomy, Isfahan Medical University, Isfahan, Iran;Department of Cell and Molecular Biology, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran iotechnology, ACECR, Isfahan, Iran;Department of Cell and Molecular Biology, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran;Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran nimal Biotechnology, ACECR, Isfahan, Iran;Isfahan Fertility and Infertility Center, Isfahan, Iran;

关键词: varicocele;    hspa2;    protamines;    dna fragmentation;    surgery;   

DOI  :  10.22074/ijfs.2010.45805

来源: DOAJ

【 摘 要 】

Background Heat shock protein A2 (HSPA2) is correlated with sperm maturity and function. Therefore, dysfunctional expression of this gene results in abnormal spermatogenesis. On the other hand, DNA damage in spermatozoa is considered to be an important cause of male infertility, and the presence of sperm with DNA fragmentation and chromatin abnormalities in human ejaculates is well documented, in particular in men with poor semen quality. Therefore, the aim of this study is to evaluate HSPA2 expression and its relation with DNA fragmentation, protamine deficiency involved in DNA packaging and semen parameters in varicocele patients in comparison to fertile men before and after varicocelectomy. Materials and methods This study included 52 fertile individuals as the control group and 70 infertile individuals with varicocele as the experimental group. Sperm DNA fragmentation, protamine deficiency and relative HSPA2 expression were evaluated by the sperm chromatin dispersion test, chromomycin A3 staining and RT-PCR, respectively. Results The mean values of abnormal morphology, protamine deficiency and DNA fragmentation were significantly lower in varicocele individuals following varicocelectomy when compared to fertile individuals. The correlation between these parameters were studied and discussed in the text. Conclusion There is a decrease in relative HSPA2 expression which is possibly due to chronic induced hyperthermia in varicocele individuals. Removal of this stress increases HSPA2 expression and results in the proper folding of proteins involved in spermatogenesis; therefore resulting in improved DNA packaging, as well as better sperm morphology and motility which may indirectly reduce sperm DNA fragmentation.

【 授权许可】

Unknown