Distinct fatty acid signatures in infrapatellar fat pad and synovial fluid of patients with osteoarthritis versus rheumatoid arthritis

Arthritis Research & Therapy

Sanna Turunen¹  Johanna Huhtakangas¹  Petri Lehenkari¹  Antti Joukainen²  Tommi Kääriäinen²  Heikki Kröger²  Tommi Paakkonen³  Anne-Mari Mustonen³  Petteri Nieminen³  Reijo Käkelä⁴ 

[1] Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu;Department of Orthopaedics, Traumatology and Hand Surgery, Kuopio University Hospital (KYS);Institute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland;Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki;

关键词: Fatty acid;    Infrapatellar fat pad;    n-3 polyunsaturated fatty acids;    n-6 polyunsaturated fatty acids;    Osteoarthritis;    Rheumatoid arthritis;   

DOI  :  10.1186/s13075-019-1914-y

来源: DOAJ

【 摘 要 】

Abstract Background Infrapatellar fat pad (IFP) has recently emerged as a potential source of inflammation in knee arthropathies. It has been proposed to be one source of adipocytokines, fatty acids (FA), and FA-derived lipid mediators that could contribute to the pathophysiological processes in the knee joint. Alterations in synovial fluid (SF) lipid composition have been linked to both osteoarthritis (OA) and rheumatoid arthritis (RA). The aim of the present study was to compare the FA signatures in the IFP and SF of RA and OA patients. Methods Pairs of IFP and SF samples were collected from the same knees of RA (n = 10) and OA patients (n = 10) undergoing total joint replacement surgery. Control SF samples (n = 6) were harvested during diagnostic or therapeutic arthroscopic knee surgery unrelated to RA or OA. The FA composition in the total lipids of IFP and SF was determined by gas chromatography with flame ionization and mass spectrometric detection. Results Arthropathies resulted in a significant reduction in the SF proportions of n-6 polyunsaturated FA (PUFA), more pronouncedly in OA than in RA. OA was also characterized with reduced percentages of 22:6n-3 and lower product/precursor ratios of n-3 PUFA. The proportions of total monounsaturated FA increased in both RA and OA SF. Regarding IFP, RA patients had lower proportions of 20:4n-6, total n-6 PUFA, and 22:6n-3, as well as lower product/precursor ratios of n-3 PUFA compared to OA patients. The average chain length of SF FA decreased in both diagnoses and the double bond index in OA. Conclusions The observed complex alterations in the FA signatures could have both contributed to but also limited the inflammatory processes and cartilage destruction in the RA and OA knees.

【 授权许可】

Unknown