Distinct fatty acid signatures in infrapatellar fat pad and synovial fluid of patients with osteoarthritis versus rheumatoid arthritis

Arthritis Research & Therapy

Tommi Paakkonen¹  Anne-Mari Mustonen²  Petteri Nieminen²  Sanna Turunen³  Petri Lehenkari³  Johanna Huhtakangas⁴  Reijo Käkelä⁵  Antti Joukainen⁶  Heikki Kröger⁶  Tommi Kääriäinen⁶ 

[1] 0000 0001 0726 2490, grid.9668.1, Institute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland;0000 0001 0726 2490, grid.9668.1, Institute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland;0000 0001 0726 2490, grid.9668.1, Department of Environmental and Biological Sciences, Faculty of Science and Forestry, University of Eastern Finland, P.O. Box 111, FI-80101, Joensuu, Finland;0000 0001 0941 4873, grid.10858.34, Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, P.O. Box 5000, FI-90014, Oulu, Finland;0000 0004 4685 4917, grid.412326.0, Department of Surgery and Medical Research Center, Oulu University Hospital (OYS), P.O. Box 21, FI-90029, Oulu, Finland;0000 0001 0941 4873, grid.10858.34, Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, P.O. Box 5000, FI-90014, Oulu, Finland;0000 0004 4685 4917, grid.412326.0, Rheumatology Unit and Medical Research Center, Oulu University Hospital (OYS), P.O. Box 21, FI-90029, Oulu, Finland;0000 0004 0410 2071, grid.7737.4, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, P.O. Box 65, FI-00014, Helsinki, Finland;0000 0004 0410 2071, grid.7737.4, Helsinki University Lipidomics Unit (HiLIPID), Helsinki Institute for Life Science (HiLIFE), University of Helsinki, P.O. Box 65, FI-00014, Helsinki, Finland;0000 0004 0628 207X, grid.410705.7, Department of Orthopaedics, Traumatology and Hand Surgery, Kuopio University Hospital (KYS), P.O. Box 100, FI-70029, Kuopio, Finland;

关键词: Fatty acid;    Infrapatellar fat pad;    n-3 polyunsaturated fatty acids;    n-6 polyunsaturated fatty acids;    Osteoarthritis;    Rheumatoid arthritis;    Synovial fluid;   

DOI  :  10.1186/s13075-019-1914-y

来源: publisher

【 摘 要 】

BackgroundInfrapatellar fat pad (IFP) has recently emerged as a potential source of inflammation in knee arthropathies. It has been proposed to be one source of adipocytokines, fatty acids (FA), and FA-derived lipid mediators that could contribute to the pathophysiological processes in the knee joint. Alterations in synovial fluid (SF) lipid composition have been linked to both osteoarthritis (OA) and rheumatoid arthritis (RA). The aim of the present study was to compare the FA signatures in the IFP and SF of RA and OA patients.MethodsPairs of IFP and SF samples were collected from the same knees of RA (n = 10) and OA patients (n = 10) undergoing total joint replacement surgery. Control SF samples (n = 6) were harvested during diagnostic or therapeutic arthroscopic knee surgery unrelated to RA or OA. The FA composition in the total lipids of IFP and SF was determined by gas chromatography with flame ionization and mass spectrometric detection.ResultsArthropathies resulted in a significant reduction in the SF proportions of n-6 polyunsaturated FA (PUFA), more pronouncedly in OA than in RA. OA was also characterized with reduced percentages of 22:6n-3 and lower product/precursor ratios of n-3 PUFA. The proportions of total monounsaturated FA increased in both RA and OA SF. Regarding IFP, RA patients had lower proportions of 20:4n-6, total n-6 PUFA, and 22:6n-3, as well as lower product/precursor ratios of n-3 PUFA compared to OA patients. The average chain length of SF FA decreased in both diagnoses and the double bond index in OA.ConclusionsThe observed complex alterations in the FA signatures could have both contributed to but also limited the inflammatory processes and cartilage destruction in the RA and OA knees.

【 授权许可】

CC BY