Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Synthetic and Systems Biotechnology

Zachary Z. Sun¹  Abel C. Chiao²  Matthew W. Lux²  Stephanie D. Cole³  Aleksandr E. Miklos³ 

[1] Synvitrobio Inc., San Francisco, CA, USA;Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA;US Army Combat Capabilities Development Command Chemical Biological Center, 8567 Ricketts Point Road, Aberdeen Proving Ground, MD, 21010, USA;

关键词: Cell-free systems;    Cell-free expression;    Cell-free extract;    Synthetic biology;    Methods;   

DOI  :  

来源: DOAJ

【 摘 要 】

Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review of cell-extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the diversity of Escherichia coli genetic strains available and their corresponding utility. We then trace the history of cell-extract methodology over the past 20 years, showing key improvements that lower the entry level for new researchers. Next, we survey the rise of new prokaryotic cell-free systems, with associated methods, and the opportunities provided. Finally, we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible.

【 授权许可】

Unknown