Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

Microorganisms

DongChan Moon¹  Suk-Kyung Lim¹  HeeYoung Kang¹  Soon-Seek Yoon¹  MiHyun Kim¹  Ji-Hyun Choi¹  Su-Jeong Kim¹  AbrahamFikru Mechesso¹  Hyun-Ju Song¹ 

[1] Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Korea;

关键词: broiler;    β-lactamase;    bla_CTX-M;    bla_CMY-2;    E. coli;    mcr-1;   

DOI  :  10.3390/microorganisms8091434

来源: DOAJ

【 摘 要 】

We aimed to identify and characterize extended-spectrum β-lactamase (ESBL)-and/or plasmid-mediated AmpC β-lactamase (pAmpC)-producing Escherichia coli isolated from healthy broiler chickens slaughtered for human consumption in Korea. A total of 332 E. coli isolates were identified from 339 cloacal swabs in 2019. More than 90% of the isolates were resistant to multiple antimicrobials. ESBL/pAmpC-production was noted in 14% (46/332) of the isolates. Six of the CTX-M-β-lactamase-producing isolates were found to co-harbor at least one plasmid-mediated quinolone resistance gene. We observed the co-existence of bla_CMY-2 and mcr-1 genes in the same isolate for the first time in Korea. Phylogenetic analysis demonstrated that the majority of bla_CMY-2-carrying isolates belonged to subgroup D. Conjugation confirmed the transferability of bla_CTX-M and bla_CMY-2 genes, as well as non-β-lactam resistance traits from 60.9% (28/46) of the ESBL/pAmpC-producing isolates to a recipient E. coli J53. The ISECP, IS903, and orf477 elements were detected in the upstream or downstream regions. The bla_CTX-M and bla_CMY-2 genes mainly belonged to the IncI1, IncHI2, and/or IncFII plasmids. Additionally, the majority of ESBL/pAmpC-producing isolates exhibited heterogeneous PFGE profiles. This study showed that healthy chickens act as reservoirs of ESBL/pAmpC-producing E. coli that can potentially be transmitted to humans.

【 授权许可】

Unknown