Emergence of Salmonella enterica Serovar Infantis Harboring IncI1 Plasmid with blaCTX-M-14 in a Broiler Farm in Japan

Journal of Veterinary Medical Science

Mitsuhiro KAMEYAMA³  Takehisa CHUMA²  Junko YABATA³  Kiyoshi TOMINAGA³  Karoku OKAMOTO²  Hiroyuki IWATA¹  Tomoki YOKOI²  Daisuke MIYASAKO² 

[1] Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan;Laboratory of Veterinary Public Health, Joint Faculty of Veterinary Medicine, Kagoshima University, 1–21–24 Korimoto, Kagoshima 890–0065, Japan;Yamaguchi Prefectural Institute of Public Health and Environment, 2–5–67 Aoi, Yamaguchi 753–0821, Japan

关键词: β-lactamase;    broiler;    CTX-M-14;    extended-spectrum plasmid replicon typing;    Salmonella Infantis;   

DOI  :  10.1292/jvms.11-0488

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(1)Cefotaxime (CTX)-resistant and -susceptible Salmonella enterica serovar Infantis isolates obtained from broilers raised on a farm in January 2010 in Japan were characterized to establish their resistance determinants. The CTX-resistant isolates produced CTX-M-14 extended-spectrum β-lactamase and harbored 2 distinct plasmid of approximately 140- and 95-kb, whereas the CTX-susceptible isolates harbored one 140-kb plasmid. The 95-kb plasmids were replicon typed as IncI1 carrying the blaCTX-M-14 gene, while the 140-kb plasmids were IncP and harbored the aphA1, aadA1, tetA, and sul1 genes. Genetic fingerprinting by pulsed-field gel electrophoresis revealed similar macrorestriction profiles amongst CTX-resistant and susceptible isolates, suggesting a clonal relationship. The presence of CTX-resistant S. Infantis on a broiler farm has occurred through the acquisition of IncI1 resistance plasmid.

【 授权许可】

Unknown