Characterization of Extended-Spectrum Cephalosporin (ESC) Resistance in Salmonella Isolated from Chicken and Identification of High Frequency Transfer of blaCMY-2 Gene Harboring Plasmid In Vitro and In Vivo

Animals

Jun-Feng Zhang¹  Ke Shang¹  Bo-Ram Kwon¹  Min Kang¹  Se-Yeoun Cha¹  Hyung-Kwan Jang¹  Bai Wei¹ 

[1] Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Center for Poultry Diseases Control, Jeonbuk National University, Iksan 54596, Korea;

关键词: Salmonella;    chicken;    extended-spectrum cephalosporin;    bla_CMY-2;    mouse;    frequent transfer;   

DOI  :  10.3390/ani11061778

来源: DOAJ

【 摘 要 】

A total of 136 Salmonella isolates from chicken feces and meat samples of the top 12 integrated chicken production companies throughout Korea were collected. Among the 17 ESC-resistant Salmonella; bla_CTX-M-15 was the most prevalent gene and two strains carried bla_TEM-1/bla_CTX-M-15 and bla_CMY-2, respectively. The transferable bla_CTX-M-15 gene was carried by IncFII plasmid in three isolates and the bla_CMY-2 gene carried by IncI1 plasmid in one isolate. bla_CMY-2 gene-harboring strain was selected as the donor based on the high frequency of bla_CMY-2 gene transfer in vitro and its transfer frequencies were determined at 10⁻³ transconjugants per recipient. The transfer of bla_CMY-2 gene-harboring plasmid derived from chicken isolate into a human pathogen; enteroinvasive Escherichia coli (EIEC), presented in mouse intestine with about 10⁻¹ transfer frequency without selective pressure. From the competition experiment; bla_CMY-2 gene-harboring transconjugant showed variable fitness burden depends on the parent strains. Our study demonstrated direct evidence that the bla_CMY-2 gene harboring Salmonella from chicken could frequently transfer its ESC-resistant gene to E. coli in a mouse intestine without antimicrobial pressure; resulting in the emergence of multidrug resistance in potentially virulent EIEC isolates of significance to human health; which can increase the risk of therapeutic inadequacy or failures.

【 授权许可】

Unknown