Human dental pulp pluripotent-like stem cells promote wound healing and muscle regeneration

Stem Cell Research & Therapy

Aernout Luttun¹  Ester Martínez-Sarrಠ Raquel Núñez-Toldrಠ Sheyla Montori²  Maher Atari²  Carlos Gil-Recio²  Domiziana Costamagna³  Maurilio Sampaolesi³  Alessio Rotini³ 

[1] Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven;Regenerative Medicine Research Institute, Universitat Internacional de Catalunya;Translational Cardiomyology Laboratory, Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven;

关键词: Dental pulp;    Stem cells;    Revascularisation;    Angiogenesis;    Wound healing;    Muscular dystrophy;   

DOI  :  10.1186/s13287-017-0621-3

来源: DOAJ

【 摘 要 】

Abstract Background Dental pulp represents an easily accessible autologous source of adult stem cells. A subset of these cells, named dental pulp pluripotent-like stem cells (DPPSC), shows high plasticity and can undergo multiple population doublings, making DPPSC an appealing tool for tissue repair or maintenance. Methods DPPSC were harvested from the dental pulp of third molars extracted from young patients. Growth factors released by DPPSC were analysed using antibody arrays. Cells were cultured in specific differentiation media and their endothelial, smooth and skeletal muscle differentiation potential was evaluated. The therapeutic potential of DPPSC was tested in a wound healing mouse model and in two genetic mouse models of muscular dystrophy (Scid/mdx and Sgcb-null Rag2-null γc-null). Results DPPSC secreted several growth factors involved in angiogenesis and extracellular matrix deposition and improved vascularisation in all three murine models. Moreover, DPPSC stimulated re-epithelialisation and ameliorated collagen deposition and organisation in healing wounds. In dystrophic mice, DPPSC engrafted in the skeletal muscle of both dystrophic murine models and showed integration in muscular fibres and vessels. In addition, DPPSC treatment resulted in reduced fibrosis and collagen content, larger cross-sectional area of type II fast-glycolytic fibres and infiltration of higher numbers of proangiogenic CD206+ macrophages. Conclusions Overall, DPPSC represent a potential source of stem cells to enhance the wound healing process and slow down dystrophic muscle degeneration.

【 授权许可】

Unknown