Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase

eLife

Florian Raible¹  Markus Hartl²  Dorothea Anrather³  Stephanie C Bannister³  Arndt von Haeseler⁴  Bui Quang Minh⁴  Fritz J Sedlazeck⁵  Sven Schenk⁵  Andrea Bileck⁵  Kristin Tessmar-Raible⁵  Christopher Gerner⁶ 

[1] Department of Analytical Chemistry, University of Vienna, Vienna, Austria;Mass Spectrometry Facility, Max F Perutz Laboratories, Vienna, Austria;Research Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, Austria;Center of Integrative Bioinformatics Vienna, Max F Perutz Laboratories, University of Vienna, Medical University of Vienna, Vienna BioCenter, Vienna, Austria;Max F Perutz Laboratories, University of Vienna, Vienna BioCenter, Vienna, Austria;Research Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, Austria;

关键词: marine biology;    chronobiology;    development;    sexual differentiation;    proteomics;    transcriptomics;   

DOI  :  10.7554/eLife.41556

来源: DOAJ

【 摘 要 】

Many marine animals, ranging from corals to fishes, synchronise reproduction to lunar cycles. In the annelid Platynereis dumerilii, this timing is orchestrated by an endogenous monthly (circalunar) clock entrained by moonlight. Whereas daily (circadian) clocks cause extensive transcriptomic and proteomic changes, the quality and quantity of regulations by circalunar clocks have remained largely elusive. By establishing a combined transcriptomic and proteomic profiling approach, we provide first systematic insight into the molecular changes in Platynereis heads between circalunar phases, and across sexual differentiation and maturation. Whereas maturation elicits large transcriptomic and proteomic changes, the circalunar clock exhibits only minor transcriptomic, but strong proteomic regulation. Our study provides a versatile extraction technique and comprehensive resources. It corroborates that circadian and circalunar clock effects are likely distinct and identifies key molecular brain signatures for reproduction, sex and circalunar clock phase. Examples include prepro-whitnin/proctolin and ependymin-related proteins as circalunar clock targets.

【 授权许可】

Unknown