Biosynthesis and Characterization of Extracellular Silver Nanoparticles from Streptomyces aizuneusis: Antimicrobial, Anti Larval, and Anticancer Activities

Molecules

Mona A. Ashor¹  Enas A. Hassan¹  Hemmat M. Abd-Elhady¹  Abdelkader Hazem¹  Nihal El Nahhas²  Shams H. Abdel-Hafez³  Samy Selim⁴  Fayez M. Saleh⁵  Samy Sayed⁶ 

[1] Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt;Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria 21515, Egypt;Department of Chemistry, College of Science, Taif University, Taif 21944, Saudi Arabia;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72388, Saudi Arabia;Department of Medical Microbiology, Faculty of Medicine, University of Tabuk, Tabuk 71491, Saudi Arabia;Department of Science and Technology, University College-Ranyah, Taif University, Taif 21944, Saudi Arabia;

关键词: microbial synthesis;    AgNPs;    identification;    antibacterial;    antifungal;    biological activities;   

DOI  :  10.3390/molecules27010212

来源: DOAJ

【 摘 要 】

The ability of microorganisms to reduce inorganic metals has launched an exciting eco-friendly approach towards developing green nanotechnology. Thus, the synthesis of metal nanoparticles through a biological approach is an important aspect of current nanotechnology. In this study, Streptomyces aizuneusis ATCC 14921 gave the small particle of silver nanoparticles (AgNPs) a size of 38.45 nm, with 1.342 optical density. AgNPs produced by Streptomyces aizuneusis were characterized by means of UV-VIS spectroscopy and transmission electron microscopy (TEM). The UV-Vis spectrum of the aqueous solution containing silver ion showed a peak between 410 to 430. Moreover, the majority of nanoparticles were found to be a spherical shape with variables between 11 to 42 nm, as seen under TEM. The purity of extracted AgNPs was investigated by energy dispersive X-ray analysis (EDXA), and the identification of the possible biomolecules responsible for the reduction of Ag⁺ ions by the cell filtrate was carried out by Fourier Transform Infrared spectrum (FTIR). High antimicrobial activities were observed by AgNPs at a low concentration of 0.01 ppm, however, no deleterious effect of AgNPs was observed on the development and occurrence of Drosophila melanogaster phenotype. The highest reduction in the viability of the human lung carcinoma and normal cells was attained at 0.2 AgNPs ppm.

【 授权许可】

Unknown