Early-generated interneurons regulate neuronal circuit formation during early postnatal development

eLife

Shu-Qing Zhang¹  Shao-Na Jiang¹  Xiao-Yi Mao¹  Ye-Qian Xu¹  Tian-Qi Chen¹  Yong-Chun Yu¹  Lin-Yun Liu¹  Zu-Liang Yuan¹  Yinghui Fu¹  Chang-Zheng Wang¹  Jian Ma² 

[1] Jing'an District Centre Hospital of Shanghai, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, China;School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing, China;

关键词: early-generated interneurons;    neocortical synchronized activity;    synaptic transmission;    development;   

DOI  :  10.7554/eLife.44649

来源: DOAJ

【 摘 要 】

A small subset of interneurons that are generated earliest as pioneer neurons are the first cohort of neurons that enter the neocortex. However, it remains largely unclear whether these early-generated interneurons (EGIns) predominantly regulate neocortical circuit formation. Using inducible genetic fate mapping to selectively label EGIns and pseudo-random interneurons (pRIns), we found that EGIns exhibited more mature electrophysiological and morphological properties and higher synaptic connectivity than pRIns in the somatosensory cortex at early postnatal stages. In addition, when stimulating one cell, the proportion of EGIns that influence spontaneous network synchronization is significantly higher than that of pRIns. Importantly, toxin-mediated ablation of EGIns after birth significantly reduce spontaneous network synchronization and decrease inhibitory synaptic formation during the first postnatal week. These results suggest that EGIns can shape developing networks and may contribute to the refinement of neuronal connectivity before the establishment of the adult neuronal circuit.

【 授权许可】

Unknown