Developmental emergence of two-stage nonlinear synaptic integration in cerebellar interneurons

eLife

Cécile Saint-Cloment¹  Florian Rückerl¹  Therese Abrahamsson¹  David A DiGregorio¹  Ryuichi Shigemoto²  Rachel M Sherrard³  Celia Biane³  Jean Mariani³  Laurence Cathala⁴ 

[1] Department of Neuroscience, Institut Pasteur, CNRS URA 21821, 25 rue du Dr Roux, Paris, France;Institute of Science and Technology Austria, Am Campus 1, Klosterneuburg, Austria;Sorbonne Université et CNRS UMR 8256, Adaptation Biologique et Vieillissement 9 Quai 10 St Bernard, Paris, France;Sorbonne Université et CNRS UMR 8256, Adaptation Biologique et Vieillissement 9 Quai 10 St Bernard, Paris, France;Paris Brain Institute, CNRS UMR 7225 - Inserm U1127 – Sorbonne Université, Groupe 12 Hospitalier Pitié Salpêtrière 47 Boulevard de l'Hôpital, Paris, France;

关键词: interneuron;    dendritic integration;    synaptic transmission;    development;    cerebellum;    neuronal computation;    Mouse;   

DOI  :  10.7554/eLife.65954

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Synaptic transmission, connectivity, and dendritic morphology mature in parallel during brain development and are often disrupted in neurodevelopmental disorders. Yet how these changes influence the neuronal computations necessary for normal brain function are not well understood. To identify cellular mechanisms underlying the maturation of synaptic integration in interneurons, we combined patch-clamp recordings of excitatory inputs in mouse cerebellar stellate cells (SCs), three-dimensional reconstruction of SC morphology with excitatory synapse location, and biophysical modeling. We found that postnatal maturation of postsynaptic strength was homogeneously reduced along the somatodendritic axis, but dendritic integration was always sublinear. However, dendritic branching increased without changes in synapse density, leading to a substantial gain in distal inputs. Thus, changes in synapse distribution, rather than dendrite cable properties, are the dominant mechanism underlying the maturation of neuronal computation. These mechanisms favor the emergence of a spatially compartmentalized two-stage integration model promoting location-dependent integration within dendritic subunits.

【 授权许可】

CC BY