Relationship between PMN-endothelium interactions, ROS production and Beclin-1 in type 2 diabetes

Redox Biology

Victor M. Victor¹  Nadezda Apostolova²  Guillermo Mariño³  Ana Jover⁴  Francisco Canet⁴  Aranzazu M. De Marañon⁴  Zaida Abad-Jiménez⁴  Francesca Iannantuoni⁴  Pedro Díaz-Pozo⁴  Carlos Morillas⁴  Milagros Rocha⁴  Sandra López-Domènech⁴ 

[1] CIBERehd - Department of Pharmacology, University of Valencia, Valencia, Spain;CIBERehd - Department of Pharmacology, University of Valencia, Valencia, Spain;Instituto de Investigación Sanitaria del Principado de Asturias, 33011, Oviedo, Spain;Service of Endocrinology and Nutrition, University Hospital Doctor Peset, Foundation for the Promotion of Health and Biomedical Research in the Valencian Region (FISABIO), Valencia, Spain;

关键词: Type 2 diabetes;    Autophagy;    Mitochondria;    PMN-Endothelium interactions;    Oxidative stress;    ROS;   

DOI  :  

来源: DOAJ

【 摘 要 】

Type 2 diabetes is closely related to oxidative stress and cardiovascular diseases. In this study, we hypothesized that polymorphonuclear leukocytes (PMN)-endothelium interactions and autophagy are associated. We evaluated PMN-endothelial interactions, ROS production and autophagy parameters in 47 type 2 diabetic patients and 57 control subjects. PMNs from type 2 diabetic patients exhibited slower rolling velocity (p < 0.001), higher rolling flux (p < 0.001) and adhesion (p < 0.001) in parallel to higher levels of total (p < 0.05) and mitochondrial ROS (p < 0.05). When the protein expression of autophagy markers was analysed, an increase of Beclin-1 (p < 0.05), LC3I (p < 0.05), LC3II (p < 0.01) and LC3II/LC3I ratio (p < 0.05) was observed. Several correlations between ROS and leukocyte-endothelium parameters were found. Interestingly, in control subjects, an increase of Beclin-1 levels was accompanied by a decrease in the number of rolling (r = 0.561) and adhering PMNs (r = 0.560) and a rise in the velocity of the rolling PMNs (r = 0.593). In contrast, in the type 2 diabetic population, a rise in Beclin-1 levels was related to an increase in the number of rolling (r = 0.437), and adhering PMNs (r = 0.467).These results support the hypothesis that PMN-endothelium interactions, ROS levels and formation of autophagosomes, especially Beclin-1 levels, are enhanced in type 2 diabetes.

【 授权许可】

Unknown