Discordant Activity of Kaempferol Towards Dengue Virus and Japanese Encephalitis Virus

Molecules

Wannapa Sornjai¹  Janejira Jaratsittisin¹  Nitwara Wikan¹  Kanokporn Triwitayakorn¹  Chit Care¹  DuncanR. Smith¹  Atitaya Hitakarun¹ 

[1] Institute of Molecular Biosciences, Mahidol University, Salaya 73170, Thailand;

关键词: kaempferol;    antiviral activity;    dengue virus;    japanese encephalitis virus;   

DOI  :  10.3390/molecules25051246

来源: DOAJ

【 摘 要 】

Kaempferol, a plant-derived flavonoid, has been reported to have activity against Japanese encephalitis virus (JEV) in BHK-21 cells. To determine the broader utility of this compound, we initially evaluated the activity of kaempferol against JEV and dengue virus (DENV) in HEK293T/17 cells. Results showed no significant antiviral activity against either virus. We subsequently investigated the activity of kaempferol against both JEV and DENV in BHK-21 cells. Results showed a significant inhibition of JEV infection but, surprisingly, a significant enhancement of DENV infection. The effect of kaempferol on both host protein expression and transcription was investigated and both transcriptional and translational inhibitory effects were observed, although a more marked effect was observed on host cell protein expression. Markedly, while GRP78 was increased in DENV infected cells treated with kaempferol, it was not increased in JEV infected cells treated with kaempferol. These results show that cellular alteration induced by one compound can have opposite effects on viruses from the same family, suggesting the presence of distinct replication strategies for these two viruses.

【 授权许可】

Unknown