Chemistry and Antiviral Activity of Arrabidaea pulchra (Bignoniaceae)

Molecules

Geraldo Célio Brandão³  Erna G. Kroon²  Danielle E.R. Souza³  José D. Souza Filho¹ 

[1] Departamento de Química, ICEX, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31.270-901, Belo Horizonte, MG, Brazil;Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31.270-901, Belo Horizonte, MG, Brazil;Faculdade de Farmácia, Departamento de Produtos Farmacêuticos, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31.270-901, Belo Horizonte, MG, Brazil

关键词: antiviral activity;    dengue virus;    Arrabidaea pulchra;    verbascoside;    caffeoylcallerianin;   

DOI  :  10.3390/molecules18089919

来源: mdpi

【 摘 要 】

The aim of the present work was to carry out a bioguided isolation of antiviral chemical constituents from an ethanol extract of leaves from Arrabidaea pulchra (Cham.) Sandwith (EEAPL) that had shown in vitro activity in a previous screening using DNA and RNA viruses. The activity of EEPAL was evaluated against the DNA viruses Human herpesvirus 1 (HSV-1) and Vaccinia virus Western Reserve (VACV-WR) as well as against the RNA viruses Murine encephalomyocarditis virus (EMCV), and Dengue virus 2 (DENV-2) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Cytotoxicity was determined in LLCMK₂ and Vero cells and the Selectivity Indexes (SI) were calculated. The most potent effect was observed against DENV-2 (EC₅₀ 46.8 ± 1.6 µg mL⁻¹; SI 2.7). For HSV-1 and VACV-WR EC₅₀ values > 200 µg mL⁻¹ were determined, while no inhibition of the cytopathic effect was observed with EMCV. Bioguided fractionation of EEAPL by partition between immiscible solvents followed by chromatography over a Sephadex LH20 column afforded two arylpropanoid glycosides, verbascoside (AP 1) and caffeoylcalleryanin (AP 2), along with a terpenoid, ursolic acid (AP 3). AP 1 and AP 3 exhibited similar anti-DENV-2 profiles, with SI values of 3.8 and 3.1, respectively, while AP 2 was the most effective anti-DENV-2 constituent, with a SI of 20.0. Our results show that A. pulchra leaves ethanol extract (EEAPL) affords compounds with antiviral activity, mainly against DENV-2.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.