Kaempferol inhibits Pseudorabies virus replication in vitro through regulation of MAPKs and NF-κB signaling pathways

Journal of Integrative Agriculture

Xu SONG¹  Yuan-feng ZOU²  Li-xia LI²  Huai-yue HU²  Gang YE²  Chang-liang HE²  Xiao-xia LIANG²  Ren-yong JIA²  Rui WANG²  Ya-qin CHEN²  Li-zi YIN²  Zhong-qiong YIN²  Xu CHEN²  Hua-qiao TANG² 

[1] Correspondence SONG Xu, Tel: [phone];Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611100, P.R. China;

关键词: kaempferol;    antiviral activity;    Pseudorabies virus;   

DOI  :  

来源: DOAJ

【 摘 要 】

Pseudorabies virus (PRV), in the family Herpesviridae, is a pathogen of Aujeszky's disease, which causes great economic losses to the pig industry. Recent outbreaks of Pseudorabies imply that new control measures are urgently needed. The present study shows that kaempferol is a candidate drug for controlling PRV infection, as it possesses the ability to inhibit PRV replication in a dose-dependent manner in vitro. Kaempferol at a concentration of 52.40 µmol L−1 could decrease PRV-induced cell death by 90%. With an 50% inhibitory concentration (IC50) value of 25.57 µmol L−1 kaempferol was more effective than acyclovir (positive control) which has an IC50 value of 54.97 µmol L−1. A mode of action study indicated that kaempferol inhibited viral penetration and replication stages, decreasing viral loads by 4- and 30-fold, respectively. Addition of kaempferol within 16 h post infection (hpi) could significantly inhibit virus replication, and viral genome copies were decreased by almost 15-fold when kaempferol was added at 2 hpi. Kaempferol regulated the NF-κB and MAPKs signaling pathways involved in PRV infection and changed the levels of the target genes of the MAPKs (ATF-2 and c-Jun) and NF-κB (IL-1α, IL-1β and IL-2) signaling pathways. The findings of the current study suggest that kaempferol could be an alternative measure to control PRV infection.

【 授权许可】

Unknown