Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi

Iranian Journal of Basic Medical Sciences

Tehseen Nawaz¹  Yasir Raza²  Shahana Urooj Kazmi²  Saeed Khan³  Fouzia Zeeshan Khan⁴  Zulfiqar Mirani⁵ 

[1] Barrett Hodgson University, Karachi, Pakistan;Dada Bhoy Institute of Higher Education Commission, Karachi, Pakistan;Department of Microbiology, University of Karachi, Karachi, Pakistan;Dow University of Health Sciences, Karachi, Pakistan;Pakistan Council of Scientific & Industrial Research Laboratories Complex, Karachi, Pakistan;

关键词: Class 1 integrons;    ESBLs;    MDR E. coli;    Multidrug resistance;    Uropathogenic E. coli;   

DOI  :  10.22038/ijbms.2018.28807.6966

来源: DOAJ

【 摘 要 】

Objective(s): Escherichia coli is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic E. coli, prevalence of the ESBL gene group and class 1 integrons.Materials and Methods: Clinical isolates of uropathogenic E. coli were isolated from different hospitals of Karachi. Antibiotic susceptibility test was performed by Kirby-Bauer Methods. Presence of β– lactamases genes (CTX, TEM, and SHV) and integron 1 were identified by polymerase chain reaction (PCR).Results: Out of 500, 105 isolates were identified as multi-drug resistant (MDR) uropathogenic E. coli. The subject MDR isolates showed the highest resistance to aztreonam, amoxil/ clavulanic acid, ampicillin, cotrimoxazole, ceftriaxone, cefipime, and cefuroxime. Genetic analysis showed that the majority of the MDR E. coli carry CTX M1 (57.1%) followed by TEM (33.3%) and SHV (9.5%). Moreover, 79% of MDR E. coli harbored class 1 integrons, whereas all three conserved genes for class 1 integrons were present in 58% of MDR E. coli. Conclusion: This study is helpful to provide information regarding the antibiotic susceptibility pattern, distribution ESBLs and class 1 integrons among uropathogenic E. coli.

【 授权许可】

Unknown