Immunoassay-based approaches for development of screening of chlorpyrifos

Journal of Analytical Science and Technology

Saumya Choudhary¹  Nitesh Kumar Poddar²  Arun Kumar Jain³  Noor Saba Khan³  Pallavi Saxena³  Dibyabhaba Pradhan⁴ 

[1] Biomedical Informatics Centre, National Institute of Pathology – ICMR;Department of Biosciences, Manipal University Jaipur;Environmental Toxicology Laboratory, ICMR- National Institute of Pathology;ICMR-AIIMS Computational Genomics Centre, ICMR;

关键词: Chlorpyrifos;    Gas chromatography;    Immunoassay kit;    Molecular modelling;    Organophosphorus;    Pesticides;   

DOI  :  10.1186/s40543-021-00282-6

来源: DOAJ

【 摘 要 】

Abstract Chlorpyrifos (CPF) is an extensively used organophosphate pesticide for crop protection. However, there are concerns of it contaminating the environment and human health with estimated three lakh deaths annually. Detection of CPF in blood samples holds significance to avoid severe health outcomes due to continuous exposure. The most common techniques for CPF detection are Gas chromatography (GC) and high-performance liquid chromatography (HPLC). However, these techniques might not be feasible at the community healthcare level due to high-cost instrumentation, time-consuming sample preparation protocol and skilled analysts. Therefore, rapid, effective and economical methods such as immunoassay would be imperative for CPF detection in biological samples. The vital step in immunoassay development is the design of a potent immunogen from non-immunogenic molecules. The molecular modelling protocol could assist in redesigning known CPF linkers and inserting them at different substitutable positions of CPF to get distinctive CPF derivatives. Molecular docking and binding free energy analysis can be used to identify the CPF derivatives having a better binding affinity with carrier protein compared to CPF. The top-ranked CPF derivatives based on docking score and binding energy could be ideal for synthesis and immunogen development. The present review will comprehend technological trends in immunoassay kits for detecting chlorpyrifos from biological samples.

【 授权许可】

Unknown