One left‐handed strand in DNA‐oligonucleotide complexes?

FEBS Letters

Gago, Federico¹  Richards, W.G.¹ 

[1] Physical Chemistry Laboratory, South Parks Road, Oxford OX1 3QZ, England

关键词: DNA;    supercoiled;    DNA;    left-handed;    Oligodeoxynucleotide;    Molecular modelling;   

DOI  :  10.1016/0014-5793(89)80483-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A single strand of oligonucleotide can bind to double helical DNA under certain conditions. This must involve some unwinding of the original double helix in a process leading to the formation of a three-stranded region. The free energy for such an entropically unlikely reaction may come from a change in the degree of supercoiling of the original DNA. The conformation of the triple strand is investigated here using computer graphics and molecular mechanics calculations. It is suggested that on binding the oligonucleotide (strand 3) to two paired strands (1 and 2) in a supercoiled DNA molecule, strand 2 might adopt a left-handed conformation whilst strand 1 and strand 3 pair in the normal Watson-Crick B-configuration.

【 授权许可】

Unknown