Determination of the relative configuration of 5,6,7,8‐tetrahydromethanopterin by two‐dimensional NMR spectroscopy

FEBS Letters

Thauer, R.K.²  Zirngibl, C.¹  Weber, W.¹  Schleucher, J.¹  Griesinger, C.¹  Schwörer, B.²  Egert, E.¹  Koch, U.¹ 

[1] Institute for Organic Chemistry, University of Frankfurt, Niederurseler Hang, D-W-6000 Frankfurt/M 50, Germany;Laboratory of Microbiology, Philipps University, Karl von Frisch Str., D-W-3550 Marburg Germany

关键词: 5;    6;    7;    8-Tetrahydromethanopterin;    Relative configuration;    Heteronuclear two-dimensional NMR;    Molecular modelling;    Methanogenic archaca;    Methanobacterium thermoantotrophicum;    NMR;    nuclear magnetic resonance spectroscopy;    TOCSY;    total correlation spectroscopy;    (DQF-)COSY;    (double quantum filtered) correlated spectroscopy;    ROESY;    rotating frame nuclear Overhauser enhancement spectroscopy;   

DOI  :  10.1016/0014-5793(92)81522-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relative configuration of the pterin moiety of 5,6,7,8-tetrahydromethanopterin 1, a coenzyme isolated from methanogenic archaea, has been determined by two-dimensional NMR spectroscopy of N ⁵ N ¹⁰-methenyl-5,6,7,8-tetrahydromethanopterin 2 to be rel-(6R; 7S; 11R). The complete proton resonance assignment of the pterin moiety or N ⁵ N ¹⁰-methylene-5,6,7,8-tetrahydromethanopterin 3 is described including the relative stereospecific assignment of the C(14a) methylene protons.

【 授权许可】

Unknown