Sensors | |
Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity | |
Xin Yue Chan1  Siti Nur Maisarah Norizan1  Kok-Gan Chan1  Wai-Fong Yin1  Norshazliza Ab Ghani1  | |
[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; | |
关键词: Labrenzia sp.; quorum quenching; quorum sensing; marine; seawater; rapid resolution liquid chromatography (RRLC); N-acylhomoserine lactone; N-(3-oxohexanoyl)-l-homoserine lactone; | |
DOI : 10.3390/s140711760 | |
来源: DOAJ |
【 摘 要 】
We report the degradation of quorum sensing N-acylhomoserine lactone molecules by a bacterium isolated from a Malaysian marine water sample. MALDI-TOF and phylogenetic analysis indicated this isolate BM1 clustered closely to Labrenzia sp. The quorum quenching activity of this isolate was confirmed by using a series of bioassays and rapid resolution liquid chromatography analysis. Labrenzia sp. degraded a wide range of N-acylhomoserine lactones namely N-(3-hexanoyl)-l-homoserine lactone (C6-HSL),N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-l-homoserine lactone (3-hydroxy-C6-HSL). Re-lactonisation bioassays confirmedLabrenzia sp. BM1 degraded these signalling molecules efficiently via lactonase activity. To the best of our knowledge, this is the first documentation of a Labrenzia sp. capable of degrading N-acylhomoserine lactones and confirmation of its lactonase-based mechanism of action.
【 授权许可】
Unknown