Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity

Sensors

Norshazliza Ab Ghani¹  Siti Nur Maisarah Norizan¹  Xin Yue Chan¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia;

关键词: Labrenzia sp.;    quorum quenching;    quorum sensing;    marine;    seawater;    rapid resolution liquid chromatography (RRLC);    N-acylhomoserine lactone;    N-(3-oxohexanoyl)-l-homoserine lactone;   

DOI  :  10.3390/s140711760

来源: mdpi

【 摘 要 】

We report the degradation of quorum sensing N-acylhomoserine lactone molecules by a bacterium isolated from a Malaysian marine water sample. MALDI-TOF and phylogenetic analysis indicated this isolate BM1 clustered closely to Labrenzia sp. The quorum quenching activity of this isolate was confirmed by using a series of bioassays and rapid resolution liquid chromatography analysis. Labrenzia sp. degraded a wide range of N-acylhomoserine lactones namely N-(3-hexanoyl)-l-homoserine lactone (C6-HSL), N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-l-homoserine lactone (3-hydroxy-C6-HSL). Re-lactonisation bioassays confirmed Labrenzia sp. BM1 degraded these signalling molecules efficiently via lactonase activity. To the best of our knowledge, this is the first documentation of a Labrenzia sp. capable of degrading N-acylhomoserine lactones and confirmation of its lactonase-based mechanism of action.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.