High-level de novo biosynthesis of glycosylated zeaxanthin and astaxanthin in Escherichia coli

Bioresources and Bioprocessing

Thomas Lautier¹  Congqiang Zhang¹  Aurélie Bouin¹  Xixian Chen¹  Xiaohui Lim¹ 

[1] Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR);

关键词: Carotenoids;    Metabolic engineering;    Synthetic biology;    Glycosylation;    Glucosyltransferase;    Zeaxanthin;   

DOI  :  10.1186/s40643-021-00415-0

来源: DOAJ

【 摘 要 】

Abstract Because of wide applications in food, feed, pharmaceutical and cosmetic industries, the carotenoid market is growing rapidly. Most carotenoids are hydrophobic, which limits their bioavailability. Glycosylation is a natural route that substantially increases the water solubility, as well as the bioavailability, photostability and biological activities of carotenoids. Here, we report metabolic engineering efforts (e.g., promoter and RBS engineering, optimization of carbon sources and supplementation of bottleneck genes) to produce glycosylated carotenoids in Escherichia coli. By fine-tuning the carotenoid-biosynthetic genes (crtX, crtZ and crtY), our strain produced up to 47.2 mg/L (~ 11,670 ppm) of zeaxanthin glucosides, ~ 78% of the total carotenoids produced. In another construct with mevalonate, astaxanthin pathway and crtX genes, the strain produced a mixture of carotenoid glucosides including astaxanthin and adonixanthin glucosides with a total yield of 8.1 mg/L (1774 ppm). Our work demonstrated a proof-of-concept study for the microbial biosynthesis of glycosylated carotenoids.

【 授权许可】

Unknown