Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients

STAR Protocols

Giovanna Lombardi¹  Panicos Shangaris²  Cristiano Scottಠ Nicoleta Mureanu³  Sarah Tai-MacArthur³  Athina Efthymiou⁴  Rebecca Pemberton⁴  Daniela Mastronicola⁵  Kypros H. Nicolaides⁵ 

[1] Medicine, King's College London, London, UK;;Microbial Sciences, Faculty of Life Sciences &Population Sciences, Faculty of Life Sciences and Medicine King's College London, 10th Floor North Wing St Thomas' Hospital, London SE1 7EH, UK;;Department of Women and Children's Health, School of Life Course &;Peter Gorer Department of Immunobiology, School of Immunology &

关键词: Cell Biology;    Cell isolation;    Health Sciences;    Clinical Protocol;    Immunology;    Stem Cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMate™ isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population.For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html.

【 授权许可】

Unknown