Obtaining purified human intestinal epithelia for single-cell analysis and organoid culture

STAR Protocols

Sarah A. Teichmann¹  Rasa Elmentaite²  Francesca Perrone³  Matthias Zilbauer⁴  Alexander D.B. Ross⁴ 

[1] Corresponding author;Department of Surgery, Universisty of Cambridge, Cambridge CB2 0QQ, UK;Wellcome Trust, MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 0SZ, UK;Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK;

关键词: Cell isolation;    Single Cell;    Clinical Protocol;    Stem Cells;    Organoids;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Here, we describe protocols for the preparation and dissociation of human fetal and pediatric intestinal tissue to a high-viability epithelial single-cell suspension. This epithelium-enriched single-cell suspension can then be used to generate single-cell RNA sequencing data as well as to create human intestinal organoids from both the fetal and pediatric intestine. Finally, this protocol details the dissociation of the intestinal organoids for use in single-cell analysis or passaging of organoids.For complete details on the use and execution of this protocol, please refer to Elmentaite et al. (2020).

【 授权许可】

Unknown