Isolation of five different primary cell types from a single sample of human skin

STAR Protocols

Donna Lowe¹  Sarah Felton²  Joseph Spitzer³  Howard Cohen⁴  Sylwia Kabacik⁴  Ken Raj⁵ 

[1] Corresponding author;Elizabeth House, 515 Limpsfield Road, Warlingham, CR6 9LF Surrey, UK;Oxford University Hospitals NHS Foundation Trust, Old Road, OX3 7LJ Oxford, UK;Radiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, UK Health Security Agency, Chilton, Didcot, OX11 0RQ Oxfordshire, UK;The Surgery, 62 Cranwich Road, N16 5JF London, UK;

关键词: Cell Biology;    Cell culture;    Cell isolation;    Health Sciences;    Clinical Protocol;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: We have developed a technique to isolate primary keratinocytes, melanocytes, fibroblasts, preadipocytes, and microvascular endothelial cells from an individual sample of human skin. The protocol describes step-by-step instructions for processing, cells isolation, and culture of neonatal foreskin, with adaptation for more demanding adult tissues. The availability of multiple isogenic cell types derived from individual skin samples offers the ability to investigate various areas of biology, in the context of cell-type specificity without potential confounding influence of inter-individual or genetic differences.For complete details on the use and execution of this protocol, please refer to Holliman et al. (2017), Horvath et al. (2019), Horvath et al. (2018), Kabacik et al. (2018), Lowe et al. (2020), Lu et al. (2019), and Lu et al. (2018).

【 授权许可】

Unknown