Building in vitro 3D human multicellular models of high-grade serous ovarian cancer

STAR Protocols

Oliver M.T. Pearce¹  Beatrice Malacrida²  Frances R. Balkwill² 

[1] Corresponding author;Centre for Tumour Microenvironment, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK;

关键词: Cancer;    Cell Biology;    Cell culture;    Cell isolation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Three-dimensional (3D), multicellular in vitro models provide a useful platform for studying human cancer biology, particularly through deconvolution of the tumor microenvironment, or where animal models do not recapitulate the human condition. Here, we detail a protocol for building human multicellular models made of patient-derived primary cells and malignant cell lines, which recapitulate features of the tumor microenvironment. This protocol is optimized for building 3D models of high-grade serous ovarian cancer omental metastasis but can be adapted for modeling other cancers.For complete details on the use and execution of this profile, please refer to Delaine-Smith et al. (2021) and Malacrida et al. (2021).

【 授权许可】

Unknown