Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors

STAR Protocols

Virginie Baus¹  Monika Dieterle²  Anna Golebiewska³  Georgia Kanli⁴  Rolf Bjerkvig⁴  May Wantz⁵  Olivier Keunen⁵  Anaïs Oudin⁵  Amandine Bernard⁵  Carina Fabian⁵  Simone P. Niclou⁵  Yahaya A. Yabo⁵  Eliane Klein⁵  Vanessa Barthelemy⁵  Claire Dording⁵  Alessandro Michelucci⁵  Ann-Christin Hau⁵ 

[1] Corresponding author;Department of Biomedicine, University of Bergen, 5019 Bergen, Norway;Faculty of Science, Technology and Medicine, University of Luxembourg, 4367 Belvaux, Luxembourg;Neuro-Immunology Group, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg;NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg;

关键词: Cell Biology;    Cell culture;    Cell isolation;    Cancer;    Model Organisms;    Neuroscience;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing.For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020).

【 授权许可】

Unknown