Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

STAR Protocols

Haruyoshi Yamaza¹  Soichiro Sonoda²  Koichiro Yoshimaru³  Tomoaki Taguchi⁴  Takayoshi Yamaza⁴ 

[1] Corresponding author;Department of Molecular Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka 812-8582, Japan;Department of Pediatric Dentistry, Kyushu University Graduate School of Dental Science, Fukuoka 812-8582, Japan;Department of Pediatric Surgery, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan;

关键词: Cell isolation;    Clinical Protocol;    Stem Cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays.For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).

【 授权许可】

Unknown