An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration

STAR Protocols

John H. Kehrl¹  Serena L.S. Yan² 

[1] Corresponding author;B-cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA;

关键词: Cell Biology;    Cell isolation;    Immunology;    Microscopy;    Model Organisms;    Antibody;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Actin plays a crucial role during cell motility, but the organization of F-actin filaments during lymphocyte migration has not been visualized in vivo. Here, we present a 4D imaging platform using high-resolution confocal intravital microscopy to precisely determine the F-actin filament profile during lymphocyte transendothelial migration and interstitial migration. This protocol allows prolonged live imaging by laser scanning microscopy with advanced spatial resolution compared with the traditional multi-photon intravital microscopy techniques.For complete details on the use and execution of this protocol, please refer to Yan et al. (2019).

【 授权许可】

Unknown