Isolation, culture, and immunostaining of neonatal rat ventricular myocytes

STAR Protocols

Kleber Gomes Franchini¹  Ana Helena Macedo Pereira²  Alisson Campos Cardoso² 

[1] Corresponding author;Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Sao Paulo, Brazil;

关键词: Cell Biology;    Cell culture;    Cell isolation;    Microscopy;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology in vitro. Here, we present our modified enzymatic digestion protocol followed by two-step discontinuous Percoll gradient centrifugation to isolate a high yield of viable ventricular cardiomyocytes from neonatal rats. Finally, here we describe an immunostaining protocol for cytosolic and nuclear staining of NRVMs.For complete details on the use and execution of this protocol, please refer to Pereira et al. (2020).

【 授权许可】

Unknown