Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

STAR Protocols

Rupert Beale¹  Rachel Ulferts¹  Aneesh Sharma²  Hector Huerga Encabo³  Dominique Bonnet³ 

[1] Corresponding author;Cell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK;Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK;

关键词: Cell Biology;    Cell isolation;    Cell-based Assays;    Immunology;    Microbiology;    Microscopy;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay.For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021)

【 授权许可】

Unknown