Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital

Frontiers in Microbiology

Vasanthi Pinto¹  Nilanthi Dissanayake²  Veranja Liyanapathirana²  Margaret Ip³  Norman Lo³  Carmen Li³  Mamie Hui³  Kam T. Wong³  Chendi Zhu³ 

[1] Department of Anesthesiology and Critical Care, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka;Department of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka;Department of Microbiology, The Chinese University of Hong Kong, Shatin, Hong Kong;

关键词: OXA-181;    quinolone;    yersiniabactin;    Klebsiella pneumoniae;    Sri Lanka;   

DOI  :  10.3389/fmicb.2018.02044

来源: DOAJ

【 摘 要 】

Limited data is available on the epidemiology and characteristics of carbapenem-resistant Enterobacteriaceae (CRE) and their associated plasmids or virulence determinants from Sri Lanka. Through whole genome sequencing of CREs from the intensive care units of a Sri Lankan teaching hospital, we identified a carbapenemase gene, blaOXA–181 in 10 carbapenemase-producing Klebsiella pneumoniae isolates (two strains of ST437 and eight strains of ST147) from 379 respiratory specimens. blaOXA–181 was carried in three variants of ColE-type plasmids. K. pneumoniae strains with ompK36 variants showed high minimum inhibitory concentrations to carbapenem. Furthermore, genes encoding for extended spectrum β-lactamases (ESBL), plasmid-mediated quinolone resistance (PMQR) determinants (qnr, aac(6′)-Ib-cr, and oqxAB) were present in all 10 strains. Amino acid substitution in chromosomal quinolone resistance-determining regions (QRDRs) gyrA (Ser83Ile) and parC (Ser80Ile) were also observed. All strains had yersiniabactin genes on mobile element ICEkp. Strict infection control practices and judicious use of antibiotics are warranted to prevent further spread of multidrug-resistant K. pneumoniae.

【 授权许可】

Unknown