Polystyrene nanoparticles induced neurodevelopmental toxicity in Caenorhabditis elegans through regulation of dpy-5 and rol-6

Ecotoxicology and Environmental Safety

Hui Li¹  Yingying Jin²  Jing An²  Wanlei Xue²  Yufang Zhong²  Siyan Wang²  Yu Shang²  Hongli Wang³ 

[1] Correspondence to: School of Environmental and Chemical Engineering, Shanghai University, Nanchen Road 333, Shanghai 200444, China.;School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;State Environmental Protection Key Laboratory of Formation and Prevention of Urban Complex Air Pollution, Shanghai Academy of Environment Sciences, Shanghai 200233, China;

关键词: Polystyrene nanoparticles;    Caenorhabditis elegans;    Neurodevelopmental toxicity;    Oxidative damage;   

DOI  :  

来源: DOAJ

【 摘 要 】

Micro- and nano- polystyrene particles have been widely detected in environment, posing potential threats to human health. This study was designed to evaluate the neurodevelopmental toxicity of polystyrene nanoparticles (NPs) in Caenorhabditis elegans (C. elegans), to screen crucial genes and investigate the underlying mechanism. In wild-type C. elegans, polystyrene NPs (diameter 50 nm) could concentration-dependently induce significant inhibition in body length, survival rate, head thrashes, and body bending, accompanying with increase of reactive oxygen species (ROS) production, lipofuscin accumulation, and apoptosis and decrease of dopamine (DA) contents. Moreover, pink-1 mutant was demonstrated to alleviate the locomotion disorders and oxidative damage induced by polystyrene NPs, indicating involvement of pink-1 in the polystyrene NPs-induced neurotoxicity. RNA sequencing results revealed 89 up-regulated and 56 down-regulated differently expressed genes (DEGs) response to polystyrene NPs (100 μg/L) exposure. Gene Ontology (GO) enrichment analysis revealed that predominant enriched DEGs were correlated with biological function of cuticle development and molting cycle. Furthermore, mutant strains test showed that the neurodevelopmental toxicity and oxidative stress responses induced by 50 nm polystyrene NPs were regulated by dpy-5 and rol-6. In general, polystyrene NPs induced obvious neurodevelopmental toxicity in C. elegans through oxidative damage and dopamine reduction. Crucial genes dpy-5 and rol-6 might participate in polystyrene NPs-induced neurodevelopmental toxicity through regulation on synthesis and deposition of cuticle collagen.

【 授权许可】

Unknown