In Situ Synthesis of Silver Nanoparticles in a Hydrogel of Carboxymethyl Cellulose with Phthalated-Cashew Gum as a Promising Antibacterial and Healing Agent

International Journal of Molecular Sciences

Peter Eaton¹  Antônia Carla de Jesus Oliveira¹  Adriany das Graças Nascimento Amorim¹  José Roberto de Souza de Almeida Leite¹  Ana Karina Marques Fortes Lustosa¹  Durcilene Alves da Silva¹  Patrick Veras Quelemes¹  Rita de Cássia Meneses de Oliveira²  Irisdalva Sousa Oliveira²  Francilene Vieira da Silva²  Miguel Peixoto de Almeida³  Cristina Delerue-Matos⁴  Alexandra Plácido⁴ 

[1] Center for Biodiversity Research and Biotechnology, Biotec, Federal University of Piauí, Av. São Sebastião, 2819, Reis Veloso, 64202-020 Parnaíba-PI, Brazil;Medicinal Plants Reserarch Center, NPPM, Federal University of Piauí, Campus Ministro Petrônio Portella, Bairro Ininga, 64049-550 Teresina- PI, Brazil;REQUIMTE/LAQV, Department of Chemistry and Biochemistry, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal;REQUIMTE/LAQV, Superior Engineering Institute of Porto, Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal;

关键词: silver nanoparticles;    hydrogel;    cashew gum;    healing;    wounds;    antibacterial activity;   

DOI  :  10.3390/ijms18112399

来源: DOAJ

【 摘 要 】

Silver nanoparticles have been shown to possess considerable antibacterial activity, but in vivo applications have been limited due to the inherent, but low, toxicity of silver. On the other hand, silver nanoparticles could provide cutaneous protection against infection, due to their ability to liberate silver ions via a slow release mechanism, and their broad-spectrum antimicrobial action. Thus, in this work, we describe the development of a carboxymethyl cellulose-based hydrogel containing silver nanoparticles. The nanoparticles were prepared in the hydrogel in situ, utilizing two variants of cashew gum as a capping agent, and sodium borohydride as the reducing agent. This gum is non-toxic and comes from a renewable natural source. The particles and gel were thoroughly characterized through using rheological measurements, UV-vis spectroscopy, nanoparticles tracking analysis, and transmission electron microscopy analysis (TEM). Antibacterial tests were carried out, confirming antimicrobial action of the silver nanoparticle-loaded gels. Furthermore, rat wound-healing models were used and demonstrated that the gels exhibited improved wound healing when compared to the base hydrogel as a control. Thus, these gels are proposed as excellent candidates for use as wound-healing treatments.

【 授权许可】

Unknown