STAR Protocols | |
Network analysis of TCGA and GTEx gene expression datasets for identification of trait-associated biomarkers in human cancer | |
Justin A. MacDonald1  Huey-Miin Chen2  | |
[1] Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4Z6, Canada;;Department of Biochemistry & | |
关键词: Bioinformatics; Cancer; Genomics; RNAseq; Gene Expression; Systems biology; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Summary: Advances in high-throughput sequencing technologies now yield unprecedented volumes of OMICs data with opportunities to conduct systematic data analyses and derive novel biological insights. Here, we provide protocols to perform differential-expressed gene analysis of TCGA and GTEx RNA-Seq data from human cancers, complete integrative GO and network analyses with focus on clinical and survival data, and identify differential correlation of trait-associated biomarkers.For complete details on the use and execution of this protocol, please refer to Chen and MacDonald (2021).
【 授权许可】
Unknown